CAS No. 19356-17-3

Batch: Purity:99.94
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Calcifediol Chemical Structure
Chemical Structure
Check the Calcifediol product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name (1S,3Z)-3-[(2E)-2-[(1R,3aS,7aR)-1-[(2R)-6-hydroxy-6-methylheptan-2-yl]-7a-methyl-2,3,3a,5,6,7-hexahydro-1H-inden-4-ylidene]ethylidene]-4-methylidenecyclohexan-1-ol
CAS Number 19356-17-3
Molecular Formula

C27H44O2
Molecular Weight (MW) 400.64
SMILES CC(CCCC(C)(C)O)C1CCC2C1(CCCC2=CC=C3CC(CCC3=C)O)C
InChIKey JWUBBDSIWDLEOM-DTOXIADCSA-N

Ordering Information

Biological Activity

Calcifediol is a major circulating metabolite of vitamin D3 that targets the vitamin D receptor (VDR) signaling pathway. It suppresses parathyroid hormone (PTH) secretion and PTH mRNA expression in parathyroid cells, exhibiting an ED50 of 2 nM for PTH suppression. Through VDR activation, calcifediol regulates calcium homeostasis and bone mineralization pathways, reducing PTH levels to modulate mineral metabolism downstream. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 80 mg/mL (199.68 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Ethanol 80 mg/mL
Water Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.