CAS No. 527-73-1

Batch: Purity:99.99
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Azomycin Chemical Structure
Chemical Structure
Check the Azomycin product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

CAS Number 527-73-1
Molecular Formula

C3H3N3O2

Molecular Weight (MW) 113.07
SMILES C1=CN=C(N1)[N+](=O)[O-]

Biological Activity

Azomycin (2-Nitroimidazole) is a naturally occurring nitroimidazole antimicrobial antibiotic originally isolated from Nocardia mesenterica. It exhibits broad-spectrum antibacterial activity against aerobic Gram-positive and Gram-negative bacteria, as well as anaerobic microorganisms. Under anaerobic conditions, nitroimidazole compounds undergo intracellular reductive activation, generating short-lived reactive intermediates that induce DNA damage, inhibit nucleic acid synthesis, and promote microbial cell death.

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 23 mg/mL (203.41 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Water Insoluble
Ethanol Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
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Note: Technical data last updated: Sep 1, 2026.