CAS No. 64907-22-8

Batch: Purity:
7α,25-Dihydroxycholesterol Chemical Structure
Chemical Structure
Check the 7α,25-Dihydroxycholesterol product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name (3S,7S,8S,9S,10R,13R,14S,17R)-17-[(2R)-6-hydroxy-6-methylheptan-2-yl]-10,13-dimethyl-2,3,4,7,8,9,11,12,14,15,16,17-dodecahydro-1H-cyclopenta[a]phenanthrene-3,7-diol
CAS Number 64907-22-8
Molecular Formula

C27H46O3
Molecular Weight (MW) 418.65
InChIKey BQMSKLCEWBSPPY-IKVTXIKFSA-N

Ordering Information

Biological Activity

7α,25-Dihydroxycholesterol is a potent endogenous oxysterol agonist of EBI2 (GPR183),. Activation of EBI2 triggers G protein-coupled signaling pathways, modulating intracellular calcium mobilization and cell migration. This receptor signaling orchestrates the spatial positioning and trafficking of immune cells, including B cells and dendritic cells, within secondary lymphoid tissues during humoral immune responses. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.