CAS No. 434-16-2

Batch: Purity:99.53
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7-Dehydrocholesterol Chemical Structure
Chemical Structure
Check the 7-Dehydrocholesterol product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

CAS Number 434-16-2
Molecular Formula

C27H44O
Molecular Weight (MW) 384.64
SMILES CC(C)CCCC(C)C1CCC2C1(CCC3C2=CC=C4C3(CCC(C4)O)C)C

Biological Activity

7-Dehydrocholesterol (7-DHC, Provitamin D3) is an endogenous zoosterol derivative that functions as an immediate precursor in free cholesterol biosynthesis and a precursor for vitamin D3 production. Upon ultraviolet radiation exposure in skin, 7-dehydrocholesterol undergoes photoisomerization to previtamin D3, which subsequently converts to vitamin D3 to regulate calcium homeostasis, bone mineralization, and cellular differentiation pathways. In cellular lipids, 7-dehydrocholesterol also participates in redox processes and lipid peroxidation networks. [1]

How to Prepare Stock Solution?

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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 5 mg/mL (12.99 mM) ; Warmed with 60°C water bath ; Ultrasonicated
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

Reference

[1] Holick MF, et al. Science. 1980;210(4466):203-5.

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Note: Technical data last updated: Sep 1, 2026.