CAS No. 7240-37-1
Batch:
Purity:95.12
Chemical Structure
Check the
7-Aminoactinomycin D
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
Chemical Information
| CAS Number | 7240-37-1 | |
|---|---|---|
| Molecular Formula |
C62H87N13O16 |
|
| Molecular Weight (MW) | 1270.43 |
Biological Activity
7-Aminoactinomycin D (7-AAD) is a cell-impermeant, fluorescent DNA intercalator with high affinity for guanine-cytosine (GC)-rich regions. Upon binding to double-stranded DNA, it inhibits RNA polymerase activity and blocks transcription. Due to its inability to cross intact cell membranes, 7-AAD is widely utilized in flow cytometry to distinguish non-viable or apoptotic cells with compromised plasma membranes from viable cells. [1]
Solubility
Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
25 mg/mL
(19.67 mM)
; Warmed with 60°C water bath
; Ultrasonicated
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Water | Insoluble |
| Ethanol | Insoluble |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Reference
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Note: Technical data last updated: Sep 1, 2026.