CAS No. 35920-39-9

Batch: Purity:99.86
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5'-N-Ethylcarboxamidoadenosine (NECA) Chemical Structure
Chemical Structure
Check the 5'-N-Ethylcarboxamidoadenosine (NECA) product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name (2S,3S,4R,5R)-5-(6-aminopurin-9-yl)-N-ethyl-3,4-dihydroxyoxolane-2-carboxamide
CAS Number 35920-39-9
Molecular Formula

C12H16N6O4
Molecular Weight (MW) 308.29
SMILES CCNC(=O)C1OC(C(O)C1O)[N]2C=NC3=C(N)N=CN=C23
InChIKey JADDQZYHOWSFJD-FLNNQWSLSA-N

Ordering Information

Biological Activity

5'-N-Ethylcarboxamidoadenosine (NECA) is a potent, nonselective adenosine receptor agonist targeting A1, A2A, A2B, and A3 adenosine receptors. Receptor stimulation by NECA modulates intracellular cAMP signaling and attenuates oxidative stress. Furthermore, NECA suppresses downstream inflammatory pathways, decreasing the gene expression of pro-inflammatory factors including TNF-α, IL-18, and ICAM-1 while blocking activation of the JNK-MAPK signaling cascade. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 62 mg/mL (201.1 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Water Insoluble
Ethanol Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.