CAS No. 1463-10-1
Batch:
Purity:99.93
Chemical Structure
Check the
5-Methyluridine
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
Chemical Information
| CAS Number | 1463-10-1 | |
|---|---|---|
| Molecular Formula |
C10H14N2O6 |
|
| Molecular Weight (MW) | 258.23 | |
| SMILES | CC1=CN(C(=O)NC1=O)C2C(C(C(O2)CO)O)O |
Biological Activity
5-Methyluridine (Ribothymidine) is a naturally occurring pyrimidine nucleoside that serves as a key endogenous human metabolite and modified nucleoside component in transfer RNA. Incorporation of 5-methyluridine into RNA molecules, particularly at the conserved position 54 of the T-loop in transfer RNA, plays a crucial role in maintaining transfer RNA structural stability, proper tertiary folding, and translational efficiency during protein synthesis. [1]
How to Prepare Stock Solution?
|
Concentration
Volume
Mass
|
|---|
Solubility
Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
25 mg/mL
(96.81 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Reference
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Note: Technical data last updated: Sep 1, 2026.