CAS No. 554-01-8

Batch: Purity:99.69
Check CoA
5-Methylcytosine Chemical Structure
Chemical Structure
Check the 5-Methylcytosine product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

CAS Number 554-01-8
Molecular Formula

C5H7N3O
Molecular Weight (MW) 125.13
SMILES CC1=C(NC(=O)N=C1)N

Biological Activity

5-Methylcytosine is a methylated pyrimidine nucleobase that functions as a major epigenetic mark in mammalian nuclear DNA to regulate gene transcription. The presence of this modified base modulates chromatin structure and transcription factor binding, thereby mediating targeted gene silencing, genomic imprinting, X-chromosome inactivation, and cellular differentiation across various developmental and physiological processes.[1][2]

How to Prepare Stock Solution?

Concentration Volume Mass
🧮 Molarity Calculator

Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
Water 8 mg/mL
DMSO Insoluble
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

Reference

[1] Bird A. Genes Dev. 2002;16(1):6-21.

[2] Greenberg MVC, Bourc'his D. Nat Rev Mol Cell Biol. 2019;20(10):590-607.

Jump to

Note: Technical data last updated: Sep 1, 2026.