CAS No. 838-07-3

Batch: Purity:99.49
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5-Methyl-2'-deoxycytidine Chemical Structure
Chemical Structure
Check the 5-Methyl-2'-deoxycytidine product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

CAS Number 838-07-3
Molecular Formula

C10H15N3O4
Molecular Weight (MW) 241.24
SMILES CC1=CN(C(=O)N=C1N)C2CC(C(O2)CO)O

Biological Activity

5-Methyl-2'-deoxycytidine is a naturally occurring methylated pyrimidine nucleoside and a primary epigenetic component in eukaryotic genomic DNA. It plays an essential role in modulating chromatin architecture, DNA methylation patterns, and transcriptional gene silencing across various cellular pathways. Incorporation and maintenance of this modified base regulate epigenetic inheritance, gene expression profiles, and genomic stability in mammalian systems. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 48 mg/mL (198.97 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Water 48 mg/mL
Ethanol Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

Reference

[1] Jones PA, et al. Nat Rev Genet. 2002;3(6):415-28.

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Note: Technical data last updated: Sep 1, 2026.