CAS No. 861214-33-7

Batch: Purity:99.28
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5'-Fluoroindirubinoxime Chemical Structure
Chemical Structure
Check the 5'-Fluoroindirubinoxime product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name 5-fluoro-3-(3-nitroso-1H-indol-2-yl)-1H-indol-2-ol
CAS Number 861214-33-7
Molecular Formula

C16H10FN3O2
Molecular Weight (MW) 295.27
SMILES C1=CC=C2C(=C1)C(=C(N2)C3=C(NC4=C3C=C(C=C4)F)O)N=O
InChIKey LAPHNLUPQPQSKF-UHFFFAOYSA-N

Ordering Information

Biological Activity

5'-Fluoroindirubinoxime is a synthetic indirubin derivative that acts as a potent inhibitor of FMS-like tyrosine kinase 3 (FLT3). By blocking FLT3 kinase activity, this compound impairs downstream oncogenic signal transduction through the STAT5, MAP kinase, and PI3K/AKT pathways. Consequently, FLT3 inhibition reduces cellular proliferation and promotes apoptotic cell death in FLT3-dependent hematologic malignancy models. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 59 mg/mL (199.81 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Water Insoluble
Ethanol Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.