CAS No. 69075-42-9
Batch:
Purity:99.50
Chemical Structure
Check the
5-Ethynyluridine
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
Chemical Information
| IUPAC Name | 1-[(2R,3R,4S,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-5-ethynylpyrimidine-2,4-dione | |
|---|---|---|
| CAS Number | 69075-42-9 | |
| Molecular Formula |
C11H12N2O6 |
|
| Molecular Weight (MW) | 268.22 | |
| InChIKey | QCWBIPKYTBFWHH-FDDDBJFASA-N |
Biological Activity
5-Ethynyluridine (5-EU) is a cell-permeable nucleoside analogue and metabolic RNA labeler that is biosynthetically incorporated into newly synthesized RNA transcripts during transcription. Following incorporation into cellular RNA, 5-EU enables the detection and visualization of nascent RNA synthesis and turnover via copper-catalyzed azide-alkyne click chemistry cycloaddition. This selective metabolic labeling allows sensitive tracking of global transcription dynamics, gene expression changes, and RNA metabolic pathways without interfering with DNA synthesis. [1]
How to Prepare Stock Solution?
|
Concentration
Volume
Mass
|
|---|
Solubility
Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
54 mg/mL
(201.32 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Water | 14 mg/mL |
| Ethanol | 5 mg/mL |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Reference
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Note: Technical data last updated: Sep 1, 2026.