CAS No. 50-90-8
Batch:
Purity:99.34
Chemical Structure
Check the
5-Chloro-2'-deoxyuridine
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
| IUPAC Name | 5-chloro-1-[(2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]pyrimidine-2,4-dione | |
|---|---|---|
| CAS Number | 50-90-8 | |
| Molecular Formula |
C9H11ClN2O5 |
|
| Molecular Weight (MW) | 262.65 | |
| InChIKey | NJCXGFKPQSFZIB-RRKCRQDMSA-N |
5-Chloro-2'-deoxyuridine (CldU) is a halogenated thymine analog and nucleoside antimetabolite used to investigate hypochlorous acid-induced DNA damage. Following intracellular phosphorylation, CldU is incorporated into DNA during replication, where 5-chlorouracil residues induce G:C to A:T transition mutations and prompt base excision repair pathways. Additionally, its monophosphate derivative inhibits thymidylate synthase, driving uracil incorporation into DNA and uracil-DNA glycosylase-mediated cytotoxicity.[1][2]
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Concentration
Volume
Mass
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Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
53 mg/mL
(201.78 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Water | 53 mg/mL |
| Ethanol | 19 mg/mL |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Note: Technical data last updated: Sep 1, 2026.