CAS No. 582-60-5

Batch: Purity:99.45
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5,6-Dimethylbenzimidazole Chemical Structure
Chemical Structure
Check the 5,6-Dimethylbenzimidazole product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

CAS Number 582-60-5
Molecular Formula

C9H10N2
Molecular Weight (MW) 146.19
SMILES CC1=CC2=C(C=C1C)N=C[NH]2

Biological Activity

5,6-Dimethylbenzimidazole (5,6-DMB) is an endogenous precursor and lower axial ligand component of cobalamin (vitamin B12). In microorganisms, 5,6-DMB is generated from reduced flavin mononucleotide (FMNH2) by the oxygen-dependent enzyme BluB. Incorporation of 5,6-DMB into the cobalamin structure is essential for assembling functional vitamin B12, supporting crucial bacterial metabolic pathways including methionine biosynthesis and one-carbon metabolism. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 29 mg/mL (198.37 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Ethanol 29 mg/mL
Water 5 mg/mL
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

Reference

[1] Taga ME, et al. Nature. 2007;446(7134):449-53.

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Note: Technical data last updated: Sep 1, 2026.