CAS No. 13957-31-8
Batch:
Purity:99.86
Chemical Structure
Check the
4-Thiouridine
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
| IUPAC Name | 1-[(2R,3R,4S,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-4-sulfanylidenepyrimidin-2-one | |
|---|---|---|
| CAS Number | 13957-31-8 | |
| Molecular Formula |
C9H12N2O5S |
|
| Molecular Weight (MW) | 260.27 | |
| InChIKey | ZLOIGESWDJYCTF-XVFCMESISA-N |
4-Thiouridine is a thiouridine nucleoside analogue that functions as a metabolic RNA label, affinity probe, and antimetabolite. Upon cellular incorporation into nascent transcripts, it enables dynamic profiling of transcription and RNA decay kinetics. Higher concentrations of 4-thiouridine inhibit ribosomal RNA (rRNA) synthesis, triggering a nucleolar stress response and altering post-transcriptional RNA processing. [1][2]
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Concentration
Volume
Mass
|
|---|
Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
52 mg/mL
(199.79 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Water | 52 mg/mL |
| Ethanol | 13 mg/mL |
| DMSO |
52 mg/mL
(199.79 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Water | 52 mg/mL |
| Ethanol | 3 mg/mL |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Note: Technical data last updated: Sep 1, 2026.