CAS No. 56-57-5

Batch: Purity:99.93
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4-Nitroquinoline 1-oxide Chemical Structure
Chemical Structure
Check the 4-Nitroquinoline 1-oxide product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name 4-nitro-1-oxidoquinolin-1-ium
CAS Number 56-57-5
Molecular Formula

C9H6N2O3
Molecular Weight (MW) 190.16
SMILES [O-][N+](=O)C1=CC=[N+]([O-])C2=CC=CC=C12
InChIKey YHQDZJICGQWFHK-UHFFFAOYSA-N

Ordering Information

Biological Activity

4-Nitroquinoline 1-oxide (4-NQO) is a synthetic carcinogen and mutagenic chemical agent that forms bulky purine adducts with DNA. Intracellular reduction generates reactive metabolites that bind guanine and adenine residues, disrupting DNA replication and activating DNA damage response pathways. In animal models, 4-NQO administration drives multistep oral carcinogenesis, reproducing histopathological changes from pre-malignant dysplasia to invasive oral squamous cell carcinoma. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 38 mg/mL (199.83 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Ethanol 5 mg/mL
Water Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.