CAS No. 3459-18-5

Batch: Purity:99.04
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4-Nitrophenyl-2-acetamido-2-deoxy-β-D-glucopyranoside Chemical Structure
Chemical Structure
Check the 4-Nitrophenyl-2-acetamido-2-deoxy-β-D-glucopyranoside product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

CAS Number 3459-18-5
Molecular Formula

C14H18N2O8
Molecular Weight (MW) 342.30

Biological Activity

4-Nitrophenyl-2-acetamido-2-deoxy-β-D-glucopyranoside is a chromogenic substrate for N-acetyl-β-D-glucosaminidase (NAG). Upon enzymatic hydrolysis by NAG, it releases 4-nitrophenol, which produces a yellow solution measurable by spectrophotometry. This chromogenic reaction enables the rapid colorimetric quantification of NAG enzymatic activity in biological samples, serving as a functional indicator for assessing renal tubular damage, nephrotoxicity, and lysosomal hydrolase function.[1][2]

How to Prepare Stock Solution?

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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 17 mg/mL (49.66 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Water 6 mg/mL
Ethanol Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

Reference

[1] Shibata H, et al. Clin Chim Acta. 1996;251(1):53-64.

[2] Price RG. Clin Nephrol. 1992;38 Suppl 1:S14-19.

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Note: Technical data last updated: Sep 1, 2026.