CAS No. 37067-30-4

Batch: Purity:99.76
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4-Methylumbelliferyl N-acetyl-beta-D-glucosaminide Chemical Structure
Chemical Structure
Check the 4-Methylumbelliferyl N-acetyl-beta-D-glucosaminide product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

CAS Number 37067-30-4
Molecular Formula

C18H21NO8
Molecular Weight (MW) 379.36

Biological Activity

4-Methylumbelliferyl N-acetyl-beta-D-glucosaminide is a synthetic fluorogenic substrate used for the detection and assay of N-acetyl-beta-D-glucosaminidase (NAGase) activity. Upon enzymatic hydrolysis by NAGase or beta-hexosaminidase, it releases the fluorescent reporter 4-methylumbelliferone (4-MU). This fluorometric reaction enables precise evaluation of lysosomal enzyme kinetics, cell-based enzymatic assays, and biomarker signaling in renal injury and metabolic lysosomal degradation pathways.

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 76 mg/mL (200.33 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Water 3 mg/mL
Ethanol Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.