CAS No. 360-97-4

Batch: Purity:99.96
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4-Amino-5-imidazolecarboxamide Chemical Structure
Chemical Structure
Check the 4-Amino-5-imidazolecarboxamide product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

CAS Number 360-97-4
Molecular Formula

C4H6N4O
Molecular Weight (MW) 126.12
SMILES C1=NC(=C(N1)C(=O)N)N

Ordering Information

Biological Activity

4-Amino-5-imidazolecarboxamide is an imidazole derivative and key metabolic fragment, serving as a primary metabolite of the alkylating antineoplastic agents dacarbazine and 5-(3,3-bis(2-chloroethyl)-1-triazenyl)imidazole-4-carboxamide. It acts as an intermediate in purine nucleotide biosynthesis, functioning as a precursor for nucleoside and nucleotide assembly. By participating in purine salvage pathways, it modulates cellular nucleotide pool dynamics and nucleic acid synthesis in cancer cells. [1][2]

How to Prepare Stock Solution?

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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 25 mg/mL (198.22 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Water 25 mg/mL
Ethanol 25 mg/mL
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.