CAS No. 6559-91-7

Batch: Purity:99.99
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4-Demethylepipodophyllotoxin(NSC-122819,VM-26) Chemical Structure
Chemical Structure
Check the 4-Demethylepipodophyllotoxin(NSC-122819,VM-26) product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name (5S,5aR,8aR,9R)-5-hydroxy-9-(4-hydroxy-3,5-dimethoxyphenyl)-5a,6,8a,9-tetrahydro-5H-[2]benzofuro[5,6-f][1,3]benzodioxol-8-one
CAS Number 6559-91-7
Molecular Formula

C21H20O8

Molecular Weight (MW) 400.38
SMILES COC1=CC(=CC(=C1O)OC)C2C3C(COC3=O)C(C4=CC5=C(C=C24)OCO5)O
InChIKey YVCVYCSAAZQOJI-JHQYFNNDSA-N

Biological Activity

4-Demethylepipodophyllotoxin is a potent antimitotic agent that directly binds monomeric tubulin to prevent microtubule polymerization and inhibit microtubule assembly. By disrupting microtubule dynamics, it arrests cell division and inhibits tumor cellular proliferation. Additionally, treatment protects against oxidative stress by maintaining tissue levels of glutathione, catalase, and superoxide dismutase, thereby delaying tumor formation and reducing tumor incidence and volume.

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 80 mg/mL (199.81 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Water Insoluble
Ethanol Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.