CAS No. 18550-98-6
Batch:
Purity:99.94
Chemical Structure
Check the
3PO
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
Chemical Information
| IUPAC Name | (E)-3-pyridin-3-yl-1-pyridin-4-ylprop-2-en-1-one | |
|---|---|---|
| CAS Number | 18550-98-6 | |
| Molecular Formula |
C13H10N2O |
|
| Molecular Weight (MW) | 210.23 | |
| SMILES | C1=CC(=CN=C1)C=CC(=O)C2=CC=NC=C2 | |
| InChIKey | UOWGYMNWMDNSTL-ONEGZZNKSA-N |
Biological Activity
3PO is a small-molecule inhibitor of PFKFB3 with an IC50 of 22.9 μM against recombinant human protein, displaying no inhibitory activity against PFK-1. By targeting PFKFB3, 3PO suppresses glycolytic flux and significantly reduces intracellular concentrations of fructose-2,6-bisphosphate, lactate, ATP, NAD+, and NADH. This blockade of glycolysis suppresses glucose uptake and inhibits proliferation in cancer cells.
How to Prepare Stock Solution?
|
Concentration
Volume
Mass
|
|---|
Solubility
Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
42 mg/mL
(199.78 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Ethanol | 11 mg/mL |
| Water | Insoluble |
| DMSO |
42 mg/mL
(199.78 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Ethanol | 42 mg/mL |
| Water | Insoluble |
| DMSO |
42 mg/mL
(199.78 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Ethanol | 10 mg/mL |
| Water | Insoluble |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Pre-aliquoted for Longevity: Solid-state aliquots offer longer shelf life compared to stock solutions.
Looking for a specific aliquot size? Contact your local sales representative or orders@selleckchem.com.
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Note: Technical data last updated: Sep 1, 2026.