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3-Deazaneplanocin A (DZNep) Hydrochloride is a potent, dose-dependent EZH2 and histone methyltransferase inhibitor. It affects Wnt/β-catenin, PTEN/Akt, and NF-κB signaling pathways by reducing H3K27 trimethylation (H3K27me3) and modulating gene transcription, effectively inhibiting cancer cell proliferation and fibrosis while promoting apoptosis and differentiation.