CAS No. 53984-36-4

Batch: Purity:99.3
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3-chloro-5-hydroxybenzoic Acid Chemical Structure
Chemical Structure
Check the 3-chloro-5-hydroxybenzoic Acid product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name 3-chloro-5-hydroxybenzoic acid
CAS Number 53984-36-4
Molecular Formula

C7H5ClO3
Molecular Weight (MW) 172.57
SMILES C1=C(C=C(C=C1O)Cl)C(=O)O
InChIKey RJOLIYHZZKAIET-UHFFFAOYSA-N

Ordering Information

Biological Activity

3-chloro-5-hydroxybenzoic Acid is a selective agonist of the G-protein-coupled lactate receptor GPR81 (HCAR1). Activation of GPR81 by 3-chloro-5-hydroxybenzoic Acid engages Gi-mediated signaling cascades, leading to the inhibition of adenylyl cyclase and a subsequent reduction in intracellular cAMP accumulation. Downstream, this suppression of cAMP signaling effectively inhibits lipolysis in adipocytes and regulates cellular lipid metabolism pathways.

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 34 mg/mL (197.02 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Water Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.