CAS No. 85999-40-2

Batch: Purity:99.01
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23-Hydroxybetulinic acid Chemical Structure
Chemical Structure
Check the 23-Hydroxybetulinic acid product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

CAS Number 85999-40-2
Molecular Formula

C30H48O4
Molecular Weight (MW) 472.70
SMILES CC(=C)C1CCC2(C1C3CCC4C5(CCC(C(C5CCC4(C3(CC2)C)C)(C)CO)O)C)C(=O)O

Biological Activity

23-Hydroxybetulinic acid is a natural lupane-type pentacyclic triterpene isolated from Pulsatilla chinensis with antineoplastic activity. It suppresses cancer cell proliferation and triggers apoptosis through topoisomerase inhibition and reactive oxygen species-mediated mitochondrial pathways. Activation of mitochondrial apoptotic cascades leads to loss of mitochondrial membrane potential and cleavage of caspases, thereby inhibiting tumor cell viability across various human cancer cell lines.

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 94 mg/mL (198.85 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.