CAS No. 20724-73-6
Batch:
Purity:98.20
Chemical Structure
Check the
2'-C-Methylcytidine
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
Chemical Information
| IUPAC Name | 4-amino-1-[(2R,3R,4R,5R)-3,4-dihydroxy-5-(hydroxymethyl)-3-methyloxolan-2-yl]pyrimidin-2-one | |
|---|---|---|
| CAS Number | 20724-73-6 | |
| Molecular Formula |
C10H15N3O5 |
|
| Molecular Weight (MW) | 257.24 | |
| InChIKey | PPUDLEUZKVJXSZ-VPCXQMTMSA-N |
Biological Activity
2'-C-Methylcytidine (NM107) is a nucleoside analog that acts as a potent inhibitor of hepatitis C virus (HCV) NS5B RNA-dependent RNA polymerase. Upon intracellular phosphorylation to its active triphosphate form, it terminates viral RNA chain elongation, effectively suppressing HCV RNA replication in replicon cell models. Additionally, 2'-C-methylcytidine displays antiviral activity against multiple RNA viruses by disrupting viral genome replication and downstream polyprotein synthesis. [1][2]
How to Prepare Stock Solution?
|
Concentration
Volume
Mass
|
|---|
Solubility
Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
51 mg/mL
(198.25 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Water | 51 mg/mL |
| Ethanol | Insoluble |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Reference
Jump to
Note: Technical data last updated: Sep 1, 2026.