CAS No. 23945-44-0

Batch: Purity:99
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2,4-Dihydroxypyrimidine-5-carboxylic acid Chemical Structure
Chemical Structure
Check the 2,4-Dihydroxypyrimidine-5-carboxylic acid product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

CAS Number 23945-44-0
Molecular Formula

C5H4N2O4
Molecular Weight (MW) 156.10
SMILES OC(=O)C1=CNC(=O)NC1=O

Biological Activity

2,4-Dihydroxypyrimidine-5-carboxylic acid (Isoorotic acid) is an endogenous metabolite and oxidized pyrimidine derivative involved in pyrimidine metabolism and DNA base processing pathways. As a carboxylated uracil derivative, it participates in nucleic acid metabolic processes and pyrimidine base turnover, contributing to the regulation of cellular nucleotide homeostasis, nucleobase modification dynamics, and pyrimidine salvage pathways within biological systems. [1]

How to Prepare Stock Solution?

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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 31 mg/mL (198.59 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Water Insoluble
Ethanol Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

Reference

[1] Kohli RM, et al. Nature. 2013;502(7472):472-9.

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Note: Technical data last updated: Sep 1, 2026.