CAS No. 830-81-9

Batch: Purity:99.93
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1-Naphthyl acetate Chemical Structure
Chemical Structure
Check the 1-Naphthyl acetate product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

CAS Number 830-81-9
Molecular Formula

C12H10O2
Molecular Weight (MW) 186.21
SMILES CC(=O)OC1=CC=CC2=CC=CC=C21

Biological Activity

1-Naphthyl acetate is a chromogenic esterase substrate used to detect non-specific esterase and acetylcholinesterase activity. Upon enzymatic cleavage by intracellular esterases, it releases 1-naphthol, which reacts with diazonium salts to form an insoluble colored precipitate. This reaction enables the rapid cytochemical staining and identification of macrophages and monocytes, as well as the quantification of esterase activity in biological assays.

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 37 mg/mL (198.7 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.