CAS No. 70-25-7

Batch: Purity:99.94
Check CoA
MNNG (1-Methyl-3-nitro-1-nitrosoguanidine) Chemical Structure
Chemical Structure
Check the MNNG (1-Methyl-3-nitro-1-nitrosoguanidine) product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name 1-methyl-3-nitro-1-nitrosoguanidine
CAS Number 70-25-7
Molecular Formula

C2H5N5O3
Molecular Weight (MW) 147.09
SMILES CN(N=O)C(=N)N[N](=O)=O
InChIKey VZUNGTLZRAYYDE-UHFFFAOYSA-N

Biological Activity

MNNG (1-Methyl-3-nitro-1-nitrosoguanidine) is an orally active DNA alkylating agent with toxic and mutagenic properties. It exerts carcinogenic and mutagenic effects across human diploid lymphoblast lines (MIT-2, WI-L2, and GM by inducing methylating DNA damage. In vivo, MNNG is widely used to establish animal models of gastric carcinoma and precancerous lesions, which involve alterations in the PI3K/AKT/mTOR signaling pathway and gastric mucosal atrophy. [1][2]

Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 29 mg/mL (197.15 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Ethanol 29 mg/mL
Water 7 mg/mL
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

Reference

[1] Slapikoff SA, et al. Mutat Res. 1980;70(3):365-71.

[2] Zhu F, et al. Evid Based Complement Alternat Med. 2021;2021:8846813.

Jump to

Note: Technical data last updated: Sep 1, 2026.