CAS No. 5144-89-8

Batch: Purity:99.66
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1, 10-Phenanthroline monohydrate Chemical Structure
Chemical Structure
Check the 1, 10-Phenanthroline monohydrate product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name 1,10-phenanthroline;hydrate
CAS Number 5144-89-8
Molecular Formula

C12H8N2.H2O
Molecular Weight (MW) 198.22
SMILES C1=CC2=C(C3=C(C=CC=N3)C=C2)N=C1.O
InChIKey PPQJCISYYXZCAE-UHFFFAOYSA-N

Ordering Information

Biological Activity

1,10-Phenanthroline monohydrate is a classic metal-chelating bidentate ligand and inhibitor of metallopeptidases. By chelating essential divalent metal ions such as zinc, it disrupts the catalytic activity of metalloenzymes and suppresses zinc-dependent proteolytic pathways. This inhibition impairs downstream matrix metalloproteinase-mediated extracellular matrix remodeling and alters critical cellular functions dependent on metallopeptidase activity.

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 39 mg/mL (196.75 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.