YY1 Antibody [M11B11] | Primary Antibodies

Application: Reactivity: Human, Mouse, Rat, Monkey

Lane 1: Hela, Lane 2: MCF7, Lane 3: C2C12, Lane 4: COS-7

Usage Information

Dilution
WB1:1000 IP1:200 IF1:800 FCM1:100 CHIP1:50

Application
WB, IP, IF, FCM, ChIP

Reactivity
Human, Mouse, Rat, Monkey

Source
Rabbit Monoclonal Antibody

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
65 kDa

Datasheet & SDS

Biological Description

Specificity
YY1 Antibody [M11B11] detects endogenous levels of total YY1 protein.

Clone
M11B11

Synonym(s)
Transcriptional repressor protein YY1; Delta transcription factor; Yin and yang 1; YY1

Background
Yin Yang 1 (YY1) is a ubiquitously expressed zinc‑finger transcription factor that combines classical DNA binding with architectural chromatin functions to regulate a broad spectrum of genes involved in embryogenesis, differentiation, replication, and proliferation. YY1 contains four Cys‑Cys‑His‑His‑type zinc fingers that recognize specific GC‑rich motifs in promoters and enhancers, and its N‑ and C‑terminal domains facilitate recruitment of coactivators such as histone acetyltransferases and corepressors such as histone deacetylases, enabling context‑dependent activation or repression of target genes like CREB, c‑myc, p53, and histone H4. YY1 helps orchestrate three‑dimensional genome organization by promoting enhancer–promoter chromatin loops within topologically associating domains, linking distal regulatory elements to transcription initiation sites and thereby stabilizing lineage‑specific and developmental transcription programs. YY1 is essential for implantation‑stage embryos and for the expression of pluripotency factors such as Oct4 and Sox2, and its conditional ablation in neural crest and other progenitor populations leads to midembryonic lethality and severe structural defects, underscoring its role in cell‑fate decisions and tissue morphogenesis. YY1 frequently shows elevated expression and contributes to tumor growth, survival, and metastasis by modulating stem‑like properties, cell‑cycle regulators, and apoptotic pathways, and its dysregulation has been associated with poor clinical outcomes across multiple carcinomas.

Background

Yin Yang 1 (YY1) is a ubiquitously expressed zinc‑finger transcription factor that combines classical DNA binding with architectural chromatin functions to regulate a broad spectrum of genes involved in embryogenesis, differentiation, replication, and proliferation. YY1 contains four Cys‑Cys‑His‑His‑type zinc fingers that recognize specific GC‑rich motifs in promoters and enhancers, and its N‑ and C‑terminal domains facilitate recruitment of coactivators such as histone acetyltransferases and corepressors such as histone deacetylases, enabling context‑dependent activation or repression of target genes like CREB, c‑myc, p53, and histone H4. YY1 helps orchestrate three‑dimensional genome organization by promoting enhancer–promoter chromatin loops within topologically associating domains, linking distal regulatory elements to transcription initiation sites and thereby stabilizing lineage‑specific and developmental transcription programs. YY1 is essential for implantation‑stage embryos and for the expression of pluripotency factors such as Oct4 and Sox2, and its conditional ablation in neural crest and other progenitor populations leads to midembryonic lethality and severe structural defects, underscoring its role in cell‑fate decisions and tissue morphogenesis. YY1 frequently shows elevated expression and contributes to tumor growth, survival, and metastasis by modulating stem‑like properties, cell‑cycle regulators, and apoptotic pathways, and its dysregulation has been associated with poor clinical outcomes across multiple carcinomas.

References
https://pubmed.ncbi.nlm.nih.gov/16314846/ https://pubmed.ncbi.nlm.nih.gov/33102493/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%