XPF Antibody [D21K23] | Primary Antibodies

Application: Reactivity: Human, Rat, Monkey

Lane 1: Hela, Lane 2: HT-1080, Lane 3: 293T

Usage Information

Dilution
WB1:1000

Application
WB

Reactivity
Human, Rat, Monkey

Source
Rabbit Monoclonal Antibody

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
104 kDa

Datasheet & SDS

Biological Description

Specificity
XPF Antibody [D21K23] detects endogenous levels of total XPF protein.

Clone
D21K23

Synonym(s)
DNA repair endonuclease XPF; Xeroderma pigmentosum group F-complementing protein; ERCC4

Background
Excision repair cross‑complementing 4 (ERCC4, XPF) is a structure‑specific endonuclease that forms a heterodimeric complex with ERCC1, serving as a core nuclease in multiple DNA‑repair pathways, including nucleotide excision repair (NER), interstrand crosslink repair (ICLR), and certain double‑strand break (DSB)‑repair contexts. XPF contains an endonuclease domain and a helix‑helicase‑like domain that positions the ERCC1–XPF complex at DNA junctions, where it cleaves 3ʹ‑flap structures and excises the 5ʹ side of DNA lesions in cooperation with the 3ʹ endonuclease XPG during NER dual‑incision events. In NER, the ERCC1–XPF complex is recruited to damaged DNA by the XPA protein and participates in the removal of bulky, helix‑distorting lesions such as those induced by UV radiation and chemotherapeutic crosslinking agents, whereas in ICLR it is targeted to psoralen‑ and other chemically induced interstrand crosslinks through interactions with FANCD2 and the FA core complex, linking XPF activity to the Fanconi‑anemia DNA‑repair network. In DSB‑repair contexts such as single‑strand annealing, XPF and ERCC1 execute incisions at resected DNA junctions to remove non‑homologous tails and facilitate repair‑template annealing, and XPF has also been shown to persist at DNA lesions longer in repair‑deficient backgrounds.

Background

Excision repair cross‑complementing 4 (ERCC4, XPF) is a structure‑specific endonuclease that forms a heterodimeric complex with ERCC1, serving as a core nuclease in multiple DNA‑repair pathways, including nucleotide excision repair (NER), interstrand crosslink repair (ICLR), and certain double‑strand break (DSB)‑repair contexts. XPF contains an endonuclease domain and a helix‑helicase‑like domain that positions the ERCC1–XPF complex at DNA junctions, where it cleaves 3ʹ‑flap structures and excises the 5ʹ side of DNA lesions in cooperation with the 3ʹ endonuclease XPG during NER dual‑incision events. In NER, the ERCC1–XPF complex is recruited to damaged DNA by the XPA protein and participates in the removal of bulky, helix‑distorting lesions such as those induced by UV radiation and chemotherapeutic crosslinking agents, whereas in ICLR it is targeted to psoralen‑ and other chemically induced interstrand crosslinks through interactions with FANCD2 and the FA core complex, linking XPF activity to the Fanconi‑anemia DNA‑repair network. In DSB‑repair contexts such as single‑strand annealing, XPF and ERCC1 execute incisions at resected DNA junctions to remove non‑homologous tails and facilitate repair‑template annealing, and XPF has also been shown to persist at DNA lesions longer in repair‑deficient backgrounds.

References
https://pubmed.ncbi.nlm.nih.gov/30563071/ https://pubmed.ncbi.nlm.nih.gov/30165384/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%