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uPA Antibody [F9E24]

Catalog No.: F2741

Application: Reactivity: Human

Lane 1: HT1080

Usage Information

Dilution
WB1:1000 - 1:5000 IF1:50 - 1:100

Application
WB, IF

Reactivity
Human

Source
Rabbit Monoclonal Antibody

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
48 kDa 49 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Datasheet & SDS

Biological Description

Specificity
uPA Antibody [F9E24] detects endogenous levels of total uPA protein.

Clone
F9E24

Synonym(s)
Urokinase-type plasminogen activator; U-plasminogen activator; uPA; PLAU

Background
Urokinase-type plasminogen activator (uPA, PLAU) is a 411-residue secreted serine protease of the chymotrypsin family essential for pericellular proteolysis in the plasminogen activation system. Synthesized as a single-chain proenzyme (scuPA, 52 kDa) with an N-terminal growth factor domain (GFD) for uPAR binding, a kringle domain (KD) mediating fibrin and plasminogen interactions, a connecting peptide, and a catalytic serine protease domain (SPD) containing the His204-Asp256-Ser344 triad, uPA is activated by plasmin cleavage to a two-chain form (tc-uPA). By binding uPAR on cell surfaces, uPA localizes plasmin generation, leading to extracellular matrix (ECM) degradation, activation of MMPs and latent growth factors, and facilitating cell invasion and migration, all tightly regulated by PAI-1/2 inhibitors. uPA/uPAR complexes interact with integrins and vitronectin to activate ERK/MAPK, Rac1, and FAK pathways, supporting cell proliferation, survival, and chemotaxis. uPA is physiologically vital for wound healing, angiogenesis, and embryogenesis, while pathologically, it drives cancer metastasis (with high uPA levels indicating poor prognosis), atherosclerosis, and chronic inflammation via ECM remodeling.

Background

Urokinase-type plasminogen activator (uPA, PLAU) is a 411-residue secreted serine protease of the chymotrypsin family essential for pericellular proteolysis in the plasminogen activation system. Synthesized as a single-chain proenzyme (scuPA, 52 kDa) with an N-terminal growth factor domain (GFD) for uPAR binding, a kringle domain (KD) mediating fibrin and plasminogen interactions, a connecting peptide, and a catalytic serine protease domain (SPD) containing the His204-Asp256-Ser344 triad, uPA is activated by plasmin cleavage to a two-chain form (tc-uPA). By binding uPAR on cell surfaces, uPA localizes plasmin generation, leading to extracellular matrix (ECM) degradation, activation of MMPs and latent growth factors, and facilitating cell invasion and migration, all tightly regulated by PAI-1/2 inhibitors. uPA/uPAR complexes interact with integrins and vitronectin to activate ERK/MAPK, Rac1, and FAK pathways, supporting cell proliferation, survival, and chemotaxis. uPA is physiologically vital for wound healing, angiogenesis, and embryogenesis, while pathologically, it drives cancer metastasis (with high uPA levels indicating poor prognosis), atherosclerosis, and chronic inflammation via ECM remodeling.

References
https://pubmed.ncbi.nlm.nih.gov/19273310/ https://pubmed.ncbi.nlm.nih.gov/17118695/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%