Home Primary Antibodies ULK1 Antibody [A14P9]

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ULK1 Antibody [A14P9]

Catalog No.: F0312

    Application: Reactivity:
    • F0312-wb
      Lane 1: U20S, Lane 2: RD, Lane 3: C2C12, Lane 4: Vero

    Cited by 4 Publications

    Usage Information

    Dilution
    1:1000
    1:100
    Application
    WB, IP
    Reactivity
    Human, Mouse, Rat, Monkey
    Source
    Rabbit Monoclonal Antibody
    Storage Buffer
    PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3
    Storage (from the date of receipt)
    -20°C (avoid freeze-thaw cycles), 2 years
    Predicted MW
    150 kDa

    Datasheet & SDS

    Biological Description

    Specificity
    ULK1 (D8H5) Rabbit mAb detects endogenous levels of total ULK1 protein.
    Clone
    A14P9
    Synonym(s)
    Serine/threonine-protein kinase ULK1; Autophagy-related protein 1 homolog (ATG1; hATG1); Unc-51-like kinase 1; ULK1; KIAA0722
    Background
    ULK1 (Unc-51 Like Autophagy Activating Kinase 1) is a serine/threonine protein kinase belonging to the UNC-51/Atg1 family, mammalian homolog of C. elegans unc-51 and yeast Atg1, essential for autophagy initiation and neuronal axon guidance. ULK1 (1058 amino acids, ~140 kDa) comprises an N-terminal kinase domain (residues 1-280) with conserved catalytic triad, central proline/serine-rich (PS) domain (281-500) mediating protein interactions, and C-terminal domain (501-1058) with Atg13/FIP200-binding motifs and regulatory phosphorylation sites (Ser317/Ser555/Ser777 by AMPK; Ser757 by mTOR). ULK1 forms a stable complex with Atg13, FIP200, and Atg101 that localizes to ER tubulovesicular regions/Atg9 vesicles upon nutrient deprivation; AMPK phosphorylation activates ULK1 kinase activity, inducing autophosphorylation and Atg13/Atg101/FIP200 phosphorylation to nucleate phagophore assembly, while mTORC1 phosphorylation inhibits complex formation. ULK1 forms clusters of over 30 molecules to initiate autophagosome biogenesis and also has non-autophagic functions, such as mediating growth cone endocytosis for NGF trafficking (via SynGAP/syntenin) and regulating mitophagy. Dysregulation of ULK1 is implicated in neurodegeneration through axon defects, cancer, where suppressed autophagy aids tumor survival, and metabolic disorders.
    References
    • https://pubmed.ncbi.nlm.nih.gov/26921696/
    • https://pubmed.ncbi.nlm.nih.gov/29233870/

    Tech Support

    Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

    Handling Instructions

    Tel: +1-832-582-8158 Ext:3
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