TMEM111 Antibody [B9P11] | Primary Antibodies

Application: Reactivity: Human, Mouse, Rat, Pig

Usage Information

Dilution
WB1:2000-1:10000 IHC1:250-1:1000 IF1:200-1:800

Application
WB, IHC, IF

Reactivity
Human, Mouse, Rat, Pig

Source
Mouse Monoclonal Antibody

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
30 kDa 30 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Datasheet & SDS

Biological Description

Specificity
TMEM111 Antibody [B9P11] detects endogenous levels of total TMEM111 protein.

Clone
B9P11

Synonym(s)
ER membrane protein complex subunit 3; Transmembrane protein 111; EMC3; TMEM111

Background
TMEM111, or Transmembrane Protein 111, is an integral endoplasmic reticulum membrane protein of about 26 kilodaltons and is part of the TMEM family of poorly characterized multi-spanning membrane proteins that help regulate protein quality control and cellular homeostasis in tissues such as the brain, liver, and kidney. TMEM111 is a small, hydrophobic protein with multiple predicted transmembrane domains that form an intramembrane scaffold, and it has cytoplasmic loops that interact with ER-associated degradation machinery and the Bag3 chaperone complex, though its specific motifs and residues are not well-defined due to limited structural data. Its main function is to coordinate the ubiquitin-proteasome and ER-associated degradation pathways by tethering misfolded proteins to degradation complexes, facilitating their ubiquitination through E2 conjugases, and preventing toxic protein aggregation during ER stress, thus maintaining proteostasis and cell viability. TMEM111 also supports the Bag3-HSP70-HSC70 chaperone network to selectively degrade aggregation-prone proteins, connects ER stress responses to autophagy, and ensures proper protein turnover through lysosomal and proteasomal routes. When TMEM111 is dysfunctional, ER-associated degradation becomes less efficient, leading to protein accumulation implicated in diseases such as Alzheimer's, due to amyloid processing defects, and liver disorders from impaired hepatocyte homeostasis.

Background

TMEM111, or Transmembrane Protein 111, is an integral endoplasmic reticulum membrane protein of about 26 kilodaltons and is part of the TMEM family of poorly characterized multi-spanning membrane proteins that help regulate protein quality control and cellular homeostasis in tissues such as the brain, liver, and kidney. TMEM111 is a small, hydrophobic protein with multiple predicted transmembrane domains that form an intramembrane scaffold, and it has cytoplasmic loops that interact with ER-associated degradation machinery and the Bag3 chaperone complex, though its specific motifs and residues are not well-defined due to limited structural data. Its main function is to coordinate the ubiquitin-proteasome and ER-associated degradation pathways by tethering misfolded proteins to degradation complexes, facilitating their ubiquitination through E2 conjugases, and preventing toxic protein aggregation during ER stress, thus maintaining proteostasis and cell viability. TMEM111 also supports the Bag3-HSP70-HSC70 chaperone network to selectively degrade aggregation-prone proteins, connects ER stress responses to autophagy, and ensures proper protein turnover through lysosomal and proteasomal routes. When TMEM111 is dysfunctional, ER-associated degradation becomes less efficient, leading to protein accumulation implicated in diseases such as Alzheimer's, due to amyloid processing defects, and liver disorders from impaired hepatocyte homeostasis.

References
https://pubmed.ncbi.nlm.nih.gov/36930241/ https://pubmed.ncbi.nlm.nih.gov/36795401/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%