Home Primary Antibodies TIM-3 Antibody [N19P14]

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TIM-3 Antibody [N19P14]

Catalog No.: F3034

    Application: Reactivity:

    Usage Information

    Dilution
    1:1000
    1:50
    1:200 - 1:800
    1:50
    Application
    WB, IP, IHC, FCM
    Reactivity
    Human
    Source
    Rabbit Monoclonal Antibody
    Storage Buffer
    PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3
    Storage (from the date of receipt)
    -20°C (avoid freeze-thaw cycles), 2 years
    Predicted MW
    45-70 kDa

    Datasheet & SDS

    Biological Description

    Specificity
    TIM-3 Antibody [N19P14] detects endogenous levels of total TIM-3 protein.
    Clone
    N19P14
    Synonym(s)
    Hepatitis A virus cellular receptor 2; HAVcr-2; T-cell immunoglobulin and mucin domain-containing protein 3 (TIMD-3); T-cell immunoglobulin mucin receptor 3 (TIM-3); T-cell membrane protein 3; CD366; HAVCR2; TIM3; TIMD3
    Background
    TIM-3 (T cell immunoglobulin and mucin-domain containing-3) is a transmembrane glycoprotein immune checkpoint receptor expressed on T cells, NK cells, dendritic cells, and macrophages. TIM-3 contains an extracellular IgV domain for ligand binding, a mucin-like domain with extensive O-linked glycosylation, a transmembrane segment, and a cytoplasmic tail with five conserved tyrosine residues serving as signaling hubs. As an inhibitory regulator, TIM-3 suppresses Th1 immune responses and induces T cell exhaustion in chronic infection and cancer by binding ligands such as galectin-9, CEACAM-1, and HMGB1/phosphatidylserine complexes. Ligand engagement leads to tyrosine phosphorylation, Fyn kinase recruitment, Bat3 displacement, and subsequent inhibition of TCR signaling, NF-κB activation, and cytokine production (e.g., IFN-γ, TNF-α). TIM-3 also restrains innate immunity by impairing nucleic acid sensing and cross-presentation in dendritic cells. Co-expression of TIM-3 with PD-1 marks highly dysfunctional T cells in tumors, and therapeutic TIM-3 blockade can restore anti-tumor immunity, especially in combination with other checkpoint inhibitors.
    References
    • https://pubmed.ncbi.nlm.nih.gov/28258697/
    • https://pubmed.ncbi.nlm.nih.gov/32601081/

    Tech Support

    Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

    Handling Instructions

    Tel: +1-832-582-8158 Ext:3
    If you have any other enquiries, please leave a message.

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