TBC1D1 Antibody [C20L8] | Primary Antibodies

Application: Reactivity: Mouse

Lane 1: C2C12

Usage Information

Dilution
WB1:1000 IP1:50

Application
WB, IP

Reactivity
Mouse

Source
Rabbit Monoclonal Antibody

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
160 kDa

Datasheet & SDS

Biological Description

Specificity
TBC1D1 Antibody [C20L8] detects endogenous levels of total TBC1D1 protein.

Clone
C20L8

Synonym(s)
TBC1 domain family member 1; TBC1D1; KIAA1108

Background
TBC1D1 is a Rab-GTPase activating protein (Rab-GAP) enriched in skeletal muscle and closely related to AS160/TBC1D4. It serves as a key metabolic sensor coordinating glucose uptake in response to both insulin and muscle contraction by regulating GLUT4 vesicle trafficking. Insulin activates Akt, leading to phosphorylation of TBC1D1 at Thr590, which relieves Rab10 and Rab14 inhibition and enables GLUT4 exocytosis. In contrast, muscle contraction or AICAR activates AMPK, phosphorylating Ser231, Ser660, and Ser700, promoting 14-3-3 protein binding, further disinhibiting Rab-GAP activity and enhancing GLUT4 translocation independently of Akt. Contraction increases AMPK site phosphorylation, while insulin selectively targets Thr590 via Akt2. AMPKα2 inactivation significantly reduces contraction-stimulated phosphorylation at these sites, highlighting AMPK’s dominant role during exercise. This dual regulation converges on Rab10 inactivation. Phospho-null TBC1D1 mutants accelerate contraction-stimulated GLUT4 translocation, while TBC1D1 overexpression impairs insulin sensitivity. TBC1D1 is crucial for metabolic flexibility in skeletal muscle during fasting, exercise, and feeding, and is a major target for research using transgenic models or phospho-site specific antibodies, especially in studies of exercise-mimetic drugs. Loss-of-function mutations in TBC1D1 cause severe obesity and insulin resistance, and its phosphorylation pattern remains intact with high-fat diet-induced insulin resistance, underscoring its role in exercise signaling.

Background

TBC1D1 is a Rab-GTPase activating protein (Rab-GAP) enriched in skeletal muscle and closely related to AS160/TBC1D4. It serves as a key metabolic sensor coordinating glucose uptake in response to both insulin and muscle contraction by regulating GLUT4 vesicle trafficking. Insulin activates Akt, leading to phosphorylation of TBC1D1 at Thr590, which relieves Rab10 and Rab14 inhibition and enables GLUT4 exocytosis. In contrast, muscle contraction or AICAR activates AMPK, phosphorylating Ser231, Ser660, and Ser700, promoting 14-3-3 protein binding, further disinhibiting Rab-GAP activity and enhancing GLUT4 translocation independently of Akt. Contraction increases AMPK site phosphorylation, while insulin selectively targets Thr590 via Akt2. AMPKα2 inactivation significantly reduces contraction-stimulated phosphorylation at these sites, highlighting AMPK’s dominant role during exercise. This dual regulation converges on Rab10 inactivation. Phospho-null TBC1D1 mutants accelerate contraction-stimulated GLUT4 translocation, while TBC1D1 overexpression impairs insulin sensitivity. TBC1D1 is crucial for metabolic flexibility in skeletal muscle during fasting, exercise, and feeding, and is a major target for research using transgenic models or phospho-site specific antibodies, especially in studies of exercise-mimetic drugs. Loss-of-function mutations in TBC1D1 cause severe obesity and insulin resistance, and its phosphorylation pattern remains intact with high-fat diet-induced insulin resistance, underscoring its role in exercise signaling.

References
https://pubmed.ncbi.nlm.nih.gov/20701589/ https://pubmed.ncbi.nlm.nih.gov/18276596/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%