Tau Antibody [G23J10] | Primary Antibodies

Application: Reactivity: Human, Mouse, Rat

Usage Information

Dilution
WB1:1000 IHC1:300 - 1:1200 IF1:50 - 1:200

Application
WB, IHC, IF

Reactivity
Human, Mouse, Rat

Source
Rabbit Monoclonal Antibody

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
50-80 kDa

Datasheet & SDS

Biological Description

Specificity
Tau Antibody [G23J10] detects endogenous levels of total Tau protein.

Clone
G23J10

Synonym(s)
Microtubule‑associated protein tau, Neurofibrillary tangle protein, Paired helical filament‑tau (PHF‑tau), MAPT, MAPTL, MTBT1, TAU

Background
* Tau, a microtubule-associated protein expressed predominantly in neurons, belongs to a family of microtubule-stabilizing proteins that regulate cytoskeletal dynamics through isoform-specific binding to tubulin polymers. Alternative splicing of the MAPT gene produces six isoforms distinguished by N-terminal inserts, and either three or four microtubule-binding repeats in the C-terminal region, enabling differential affinity for microtubules under varying phosphorylation states. Hyperphosphorylation at multiple sites by kinases, including GSK-3β, CDK5, and ERK1/2, reduces tau's affinity for microtubules, promoting its detachment and subsequent aggregation into paired helical filaments that form neurofibrillary tangles. Tau stabilizes axonal microtubules to facilitate axonal transport of vesicles, mitochondria, and signaling molecules, with isoform balance maintaining neuronal polarity and synaptic integrity. Pathological hyperphosphorylation disrupts this stabilization, leading to microtubule disassembly, impaired trafficking, and accumulation of tau oligomers that impair synaptic function and propagate prion-like across neurons. Tau interacts with heat shock proteins such as Hsc70, which engage soluble tau following microtubule destabilisation, modulating its conformation and preventing aggregation. In Alzheimer's disease and related tauopathies, insoluble tau filaments correlate with neuronal loss and cognitive decline, and tangle pathology spreads from the entorhinal cortex to the hippocampus and neocortex.

Background

* Tau, a microtubule-associated protein expressed predominantly in neurons, belongs to a family of microtubule-stabilizing proteins that regulate cytoskeletal dynamics through isoform-specific binding to tubulin polymers. Alternative splicing of the MAPT gene produces six isoforms distinguished by N-terminal inserts, and either three or four microtubule-binding repeats in the C-terminal region, enabling differential affinity for microtubules under varying phosphorylation states. Hyperphosphorylation at multiple sites by kinases, including GSK-3β, CDK5, and ERK1/2, reduces tau's affinity for microtubules, promoting its detachment and subsequent aggregation into paired helical filaments that form neurofibrillary tangles. Tau stabilizes axonal microtubules to facilitate axonal transport of vesicles, mitochondria, and signaling molecules, with isoform balance maintaining neuronal polarity and synaptic integrity. Pathological hyperphosphorylation disrupts this stabilization, leading to microtubule disassembly, impaired trafficking, and accumulation of tau oligomers that impair synaptic function and propagate prion-like across neurons. Tau interacts with heat shock proteins such as Hsc70, which engage soluble tau following microtubule destabilisation, modulating its conformation and preventing aggregation. In Alzheimer's disease and related tauopathies, insoluble tau filaments correlate with neuronal loss and cognitive decline, and tangle pathology spreads from the entorhinal cortex to the hippocampus and neocortex.

References
https://pubmed.ncbi.nlm.nih.gov/20308058/ https://pubmed.ncbi.nlm.nih.gov/20553310/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%