SPT6 Antibody [P6D2] | Primary Antibodies

Application: Reactivity: Human, Mouse, Rat, Monkey

Lane 1: Hela, Lane 2: NIH/3T3, Lane 3: H-4-II-E, Lane 4: COS

Usage Information

Dilution
WB1:1000 IP1:200 CHIP1:50

Application
WB, IP, ChIP

Reactivity
Human, Mouse, Rat, Monkey

Source
Rabbit Monoclonal Antibody

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
210 kDa

Datasheet & SDS

Biological Description

Specificity
SPT6 Antibody [P6D2] detects endogenous levels of total SPT6 protein.

Clone
P6D2

Synonym(s)
Transcription elongation factor SPT6; SUPT6H

Background
SPT6 serves as a conserved histone chaperone and transcription elongation factor that couples RNA polymerase II activity with chromatin dynamics across eukaryotes. It organizes tandem tetratricopeptide repeats for binding phosphorylated Ser2 CTD of Rpb1 alongside an acidic N-terminal domain and SH2-like region that recruit histone H3 and processing factors like SPN1 for coordinated mRNP packaging. During transcriptional elongation, SPT6 travels with transcribing Pol II to deposit H3-H4 dimers onto newly synthesized DNA ahead of the polymerase, simultaneously retaining post-translationally modified H3 molecules through direct binding that prevents their displacement and maintains K4 methylation and K36 trimethylation marks essential for preventing cryptic initiation within gene bodies. Interaction with the Paf1 complex and Spt5 enhances Pol II processivity by facilitating nucleosome reassembly that alleviates backtracking while coordinating H3K36me3 deposition via direct recruitment of Set2 methyltransferase, thereby coupling elongation rate with downstream m6A modification and 3' end processing. SPT6 also cooperates with HDACs like Clr3 to suppress histone turnover and enforce silencing at pericentromeric repeats through retention of deacetylated H3 tails. SPT6 sustains high-fidelity gene expression during heat shock response and developmental transitions, with ubiquitous expression reflecting roles in maintaining genome stability across proliferating and differentiated states. Depletion triggers bidirectional cryptic transcription from intergenic regions and gene bodies due to nucleosome depletion, compromising mRNA quality control and viability.

Background

SPT6 serves as a conserved histone chaperone and transcription elongation factor that couples RNA polymerase II activity with chromatin dynamics across eukaryotes. It organizes tandem tetratricopeptide repeats for binding phosphorylated Ser2 CTD of Rpb1 alongside an acidic N-terminal domain and SH2-like region that recruit histone H3 and processing factors like SPN1 for coordinated mRNP packaging. During transcriptional elongation, SPT6 travels with transcribing Pol II to deposit H3-H4 dimers onto newly synthesized DNA ahead of the polymerase, simultaneously retaining post-translationally modified H3 molecules through direct binding that prevents their displacement and maintains K4 methylation and K36 trimethylation marks essential for preventing cryptic initiation within gene bodies. Interaction with the Paf1 complex and Spt5 enhances Pol II processivity by facilitating nucleosome reassembly that alleviates backtracking while coordinating H3K36me3 deposition via direct recruitment of Set2 methyltransferase, thereby coupling elongation rate with downstream m6A modification and 3' end processing. SPT6 also cooperates with HDACs like Clr3 to suppress histone turnover and enforce silencing at pericentromeric repeats through retention of deacetylated H3 tails. SPT6 sustains high-fidelity gene expression during heat shock response and developmental transitions, with ubiquitous expression reflecting roles in maintaining genome stability across proliferating and differentiated states. Depletion triggers bidirectional cryptic transcription from intergenic regions and gene bodies due to nucleosome depletion, compromising mRNA quality control and viability.

References
https://pubmed.ncbi.nlm.nih.gov/19279664/ https://pubmed.ncbi.nlm.nih.gov/23851719/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%