SMYD3 Antibody [P23D23] | Primary Antibodies

Application: Reactivity: Human, Monkey

Lane 1: HCT116, Lane 2: MCF7, Lane 3: Vero

Usage Information

Dilution
WB1:1000

Application
WB

Reactivity
Human, Monkey

Source
Rabbit Monoclonal Antibody

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
42 kDa

Datasheet & SDS

Biological Description

Specificity
SMYD3 Antibody [P23D23] detects endogenous levels of total SMYD3 protein.

Clone
P23D23

Synonym(s)
Histone-lysine N-methyltransferase SMYD3;SET and MYND domain-containing protein 3;Zinc finger MYND domain-containing protein 1;SMYD3;ZMYND1;ZNFN3A1

Background
SET and MYND domain–containing protein 3 (SMYD3) is a lysine methyltransferase of the SET‑domain family that shuttles between the nucleus and the cytoplasm and functions as a transcriptional potentiator of cancer‑promoting genes. SMYD3 binds chromatin at promoters and enhancers through its MYND domain and can methylate histone H3 at lysine 4 (H3K4), histone H4 at lysine 5 (H4K5), and possibly histone H4 at lysine 20 (H4K20), deposits that are associated both with transcriptional activation and, in some contexts, with repression through recruitment of factors such as nuclear receptor corepressor complexes, depending on the promoter architecture and interacting partners. SMYD3 methylates non‑histone proteins such as the vascular endothelial growth factor receptor 1 (VEGFR1) on lysine‑831, thereby enhancing its kinase activity and downstream signaling, and can also methylate AKT1 to promote its membrane localization and phosphoactivation, integrating methyltransferase activity with growth‑factor and metabolic‑signaling pathways. SMYD3 is frequently overexpressed in liver, breast, colorectal, and other carcinomas, and enhanced SMYD3 expression drives proliferation, adhesion, and migration of cancer cells, while knockdown or catalytic inactivation of SMYD3 suppresses growth and neoplastic phenotypes. SMYD3 exists as both a full‑length protein and a cleaved isoform lacking the N‑terminal 34 amino acids, and the truncated form shows increased methyltransferase activity toward histone H3.

Background

SET and MYND domain–containing protein 3 (SMYD3) is a lysine methyltransferase of the SET‑domain family that shuttles between the nucleus and the cytoplasm and functions as a transcriptional potentiator of cancer‑promoting genes. SMYD3 binds chromatin at promoters and enhancers through its MYND domain and can methylate histone H3 at lysine 4 (H3K4), histone H4 at lysine 5 (H4K5), and possibly histone H4 at lysine 20 (H4K20), deposits that are associated both with transcriptional activation and, in some contexts, with repression through recruitment of factors such as nuclear receptor corepressor complexes, depending on the promoter architecture and interacting partners. SMYD3 methylates non‑histone proteins such as the vascular endothelial growth factor receptor 1 (VEGFR1) on lysine‑831, thereby enhancing its kinase activity and downstream signaling, and can also methylate AKT1 to promote its membrane localization and phosphoactivation, integrating methyltransferase activity with growth‑factor and metabolic‑signaling pathways. SMYD3 is frequently overexpressed in liver, breast, colorectal, and other carcinomas, and enhanced SMYD3 expression drives proliferation, adhesion, and migration of cancer cells, while knockdown or catalytic inactivation of SMYD3 suppresses growth and neoplastic phenotypes. SMYD3 exists as both a full‑length protein and a cleaved isoform lacking the N‑terminal 34 amino acids, and the truncated form shows increased methyltransferase activity toward histone H3.

References
https://pubmed.ncbi.nlm.nih.gov/17998933/ https://pubmed.ncbi.nlm.nih.gov/26908355/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%