SHC Antibody [N2E16] | Primary Antibodies

Application: Reactivity: Mouse, Rat, Human

Usage Information

Dilution
WB1:1000-1:2000 IHC1:300

Application
WB, IHC

Reactivity
Mouse, Rat, Human

Source
Rabbit Monoclonal Antibody

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
100-140 kDa, 62 kDa 37-66 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Datasheet & SDS

Biological Description

Specificity
SHC Antibody [N2E16] detects endogenous levels of total SHC protein.

Clone
N2E16

Synonym(s)
SHC; SHCA; SHC1; SHC-transforming protein 1; SHC-transforming protein 3; SHC-transforming protein A; Src homology 2 domain-containing-transforming protein C1; SH2 domain protein C1

Background
SHC (Src homology 2 domain-containing transforming protein, or p52Shc) is an adaptor protein that plays a pivotal role in signal transduction by linking activated cell surface receptors, particularly receptor tyrosine kinases (RTKs) like EGFR, to downstream effectors without intrinsic enzymatic activity. It features a conserved N-terminal phosphotyrosine-binding (PTB) domain, a central collagen homology 1 (CH1) linker rich in phosphorylatable tyrosine residues, and a C-terminal Src homology 2 (SH2) domain, with key residues like R175 in the PTB domain enabling specific binding to phosphotyrosines on receptors. The PTB domain primarily drives cell migration, while the SH2 domain is crucial for proliferation signaling, allowing SHC to recruit adaptors like Grb2 and SOS to activate the Ras-MAPK/ERK cascade, promoting cell growth, differentiation, and survival. SHC also integrates into the PI3K/AKT pathway, relaying RTK signals for anti-apoptotic responses and is ubiquitously expressed, with high levels in the brain, heart, and melanocytes. SHC dysregulation, via overexpression or hyperphosphorylation, fuels oncogenic proliferation in cancers through sustained EGFR-Grb2-Ras interactions and contributes to cardiovascular issues like cardiac hypertrophy by modulating stress-induced growth pathways. SHC acts as a versatile scaffold in intracellular compartments, adapting signals across cytoplasmic, mitochondrial, and nuclear locales to fine-tune cellular homeostasis.

Background

SHC (Src homology 2 domain-containing transforming protein, or p52Shc) is an adaptor protein that plays a pivotal role in signal transduction by linking activated cell surface receptors, particularly receptor tyrosine kinases (RTKs) like EGFR, to downstream effectors without intrinsic enzymatic activity. It features a conserved N-terminal phosphotyrosine-binding (PTB) domain, a central collagen homology 1 (CH1) linker rich in phosphorylatable tyrosine residues, and a C-terminal Src homology 2 (SH2) domain, with key residues like R175 in the PTB domain enabling specific binding to phosphotyrosines on receptors. The PTB domain primarily drives cell migration, while the SH2 domain is crucial for proliferation signaling, allowing SHC to recruit adaptors like Grb2 and SOS to activate the Ras-MAPK/ERK cascade, promoting cell growth, differentiation, and survival. SHC also integrates into the PI3K/AKT pathway, relaying RTK signals for anti-apoptotic responses and is ubiquitously expressed, with high levels in the brain, heart, and melanocytes. SHC dysregulation, via overexpression or hyperphosphorylation, fuels oncogenic proliferation in cancers through sustained EGFR-Grb2-Ras interactions and contributes to cardiovascular issues like cardiac hypertrophy by modulating stress-induced growth pathways. SHC acts as a versatile scaffold in intracellular compartments, adapting signals across cytoplasmic, mitochondrial, and nuclear locales to fine-tune cellular homeostasis.

References
https://pubmed.ncbi.nlm.nih.gov/11607835/ https://pubmed.ncbi.nlm.nih.gov/30210578/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%