RagC Antibody [H17M14] | Primary Antibodies

Application: Reactivity: Human, Mouse, Rat, Monkey

Usage Information

Dilution
WB1:1000 IP1:50 IF1:50 - 1:200 FCM1:200 - 1:800

Application
WB, IP, IF, FCM

Reactivity
Human, Mouse, Rat, Monkey

Source
Rabbit Monoclonal Antibody

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
50 kDa

Positive Control	293 cells; 293T cells; PANC-1 cells; mIMCD-3 cells; KNRK cells; NBT-II cells; HeLa cells
Negative Control

Datasheet & SDS

Biological Description

Specificity
RagC (D8H5) Rabbit mAb detects endogenous levels of total RagC protein.

Clone
H17M14

Synonym(s)
Ras-related GTP-binding protein C; Rag C; RagC; GTPase-interacting protein 2; TIB929; RRAGC

Background
RagC is a Ras-related small GTPase from the Rag family that acts as the GDP-bound subunit in RagA or RagB (GTP)-RagC or RagD (GDP) heterodimers, which are essential for recruiting mTORC1 to lysosomes and enabling amino acid-dependent activation of cell growth and metabolism. This protein contains an N-terminal GTPase domain with switch I and II regions that shift conformation based on nucleotide binding, as well as a C-terminal Roadblock domain that interacts with Ragulator or LAMTOR complexes for lysosomal anchoring. Key residues in the interswitch helix allow allosteric communication, coordinating RagA GTP loading with the RagC GDP conformation to support RAPTOR binding. RagC stabilize the active Rag heterodimer on the lysosome via Ragulator attachment, thus facilitating mTORC1 recruitment, where Rheb-GTP then allosterically activates mTORC1 to phosphorylate targets such as S6K1, 4E-BP1, and TFEB/TFE3. This drives anabolic processes like protein synthesis, lipid production, and lysosome biogenesis, while suppressing catabolic pathways. The GDP-bound state of RagC fixes RagA’s switch regions for high-affinity docking of the RAPTOR N-terminal cassette, preserving nutrient-responsive mTORC1 signaling without altering the complex’s structure. Oncogenic mutations in RagC can lead to amino acid-independent mTORC1 hyperactivation, resembling the effects seen in tuberous sclerosis, and dysregulation of this pathway is linked to both cancer and metabolic disorders.

Background

RagC is a Ras-related small GTPase from the Rag family that acts as the GDP-bound subunit in RagA or RagB (GTP)-RagC or RagD (GDP) heterodimers, which are essential for recruiting mTORC1 to lysosomes and enabling amino acid-dependent activation of cell growth and metabolism. This protein contains an N-terminal GTPase domain with switch I and II regions that shift conformation based on nucleotide binding, as well as a C-terminal Roadblock domain that interacts with Ragulator or LAMTOR complexes for lysosomal anchoring. Key residues in the interswitch helix allow allosteric communication, coordinating RagA GTP loading with the RagC GDP conformation to support RAPTOR binding. RagC stabilize the active Rag heterodimer on the lysosome via Ragulator attachment, thus facilitating mTORC1 recruitment, where Rheb-GTP then allosterically activates mTORC1 to phosphorylate targets such as S6K1, 4E-BP1, and TFEB/TFE3. This drives anabolic processes like protein synthesis, lipid production, and lysosome biogenesis, while suppressing catabolic pathways. The GDP-bound state of RagC fixes RagA’s switch regions for high-affinity docking of the RAPTOR N-terminal cassette, preserving nutrient-responsive mTORC1 signaling without altering the complex’s structure. Oncogenic mutations in RagC can lead to amino acid-independent mTORC1 hyperactivation, resembling the effects seen in tuberous sclerosis, and dysregulation of this pathway is linked to both cancer and metabolic disorders.

References
https://pubmed.ncbi.nlm.nih.gov/20381137/ https://pubmed.ncbi.nlm.nih.gov/31601764/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%