Rab9 Antibody [C1J10] | Primary Antibodies

Application: Reactivity: Human

Usage Information

Dilution
WB1:200 IHC1:400

Application
WB, IHC

Reactivity
Human

Source
Mouse Monoclonal Antibody

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
23 kDa 25 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Positive Control	Human normal tonsil; Human spleen; Mouse adult testes tissue; HeLa cells; HEK293 cells; Jurkat cells; HepG2 cells; K562 cells; MCF7 cells; MDA-MB-231 cells
Negative Control

Datasheet & SDS

Biological Description

Specificity
Rab9 Antibody [C1J10] detects endogenous levels of total Rab9 protein.

Clone
C1J10

Synonym(s)
RAB9; RAB9A; Ras-related protein Rab-9A

Background
Rab9 is a small monomeric GTPase from the Rab subfamily within the Ras superfamily that operates mainly on late endosomes, orchestrating retrograde vesicular transport essential for lysosomal enzyme sorting and maintaining cellular homeostasis. It consists a conserved G-domain of about 180 residues organized as a six-stranded beta-sheet flanked by five alpha-helices, with GDP and GTP binding facilitated by motifs responsible for phosphate and magnesium coordination (such as the GXXXXGKS or GXXXXGKT P-loop), guanine recognition motifs (like NKXD), and Switch I and II regions that change conformation during nucleotide exchange to mediate effector interactions. The C-terminal hypervariable region undergoes prenylation for membrane anchoring, and high-resolution structural studies of the GDP-bound form have highlighted unique conformational differences in seven regions, including effector-binding sites, that set Rab9 apart from related proteins. Rab9 cycles between a GDP-bound inactive cytosolic state, complexed with GDP dissociation inhibitor, and a GTP-bound active membrane-anchored state, regulated by guanine nucleotide exchange factors and GTPase-activating proteins. In its active state, Rab9 recruits effectors such as p40 and TIP47, which tether mannose-6-phosphate receptor-coated carriers, supporting the recycling of these receptors from late endosomes to the trans-Golgi network and enabling lysosomal hydrolase reloading. Rab9 also influences late endosome size, multivesicular body formation, autophagosome-Golgi fusion, lipid transport, including glycosphingolipids, and the egress of certain pathogens such as HIV-1 and Ebola virus. Rab9 is vital for endolysosomal maturation, autophagy, and Golgi integrity, with its depletion resulting in smaller and fewer multilamellar late endosomes.

Background

Rab9 is a small monomeric GTPase from the Rab subfamily within the Ras superfamily that operates mainly on late endosomes, orchestrating retrograde vesicular transport essential for lysosomal enzyme sorting and maintaining cellular homeostasis. It consists a conserved G-domain of about 180 residues organized as a six-stranded beta-sheet flanked by five alpha-helices, with GDP and GTP binding facilitated by motifs responsible for phosphate and magnesium coordination (such as the GXXXXGKS or GXXXXGKT P-loop), guanine recognition motifs (like NKXD), and Switch I and II regions that change conformation during nucleotide exchange to mediate effector interactions. The C-terminal hypervariable region undergoes prenylation for membrane anchoring, and high-resolution structural studies of the GDP-bound form have highlighted unique conformational differences in seven regions, including effector-binding sites, that set Rab9 apart from related proteins. Rab9 cycles between a GDP-bound inactive cytosolic state, complexed with GDP dissociation inhibitor, and a GTP-bound active membrane-anchored state, regulated by guanine nucleotide exchange factors and GTPase-activating proteins. In its active state, Rab9 recruits effectors such as p40 and TIP47, which tether mannose-6-phosphate receptor-coated carriers, supporting the recycling of these receptors from late endosomes to the trans-Golgi network and enabling lysosomal hydrolase reloading. Rab9 also influences late endosome size, multivesicular body formation, autophagosome-Golgi fusion, lipid transport, including glycosphingolipids, and the egress of certain pathogens such as HIV-1 and Ebola virus. Rab9 is vital for endolysosomal maturation, autophagy, and Golgi integrity, with its depletion resulting in smaller and fewer multilamellar late endosomes.

References
https://pubmed.ncbi.nlm.nih.gov/15456905/ https://pubmed.ncbi.nlm.nih.gov/9230071/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%