PKD/PKCμ Antibody [D22N3]

Catalog No.: F3042

    Home Primary Antibodies PKD/PKCμ Antibody [D22N3]

    For research use only.

    Application: Reactivity:

    Usage Information

    Dilution
    1:1000
    1:100
    Application
    WB, IP
    Reactivity
    Human, Monkey
    Source
    Rabbit Monoclonal Antibody
    Storage Buffer
    PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3
    Storage (from the date of receipt)
    -20°C (avoid freeze-thaw cycles), 2 years
    Predicted MW
    115 kDa

    Datasheet & SDS

    Biological Description

    Specificity
    PKD/PKCμ Antibody [D22N3] detects endogenous levels of total PKD/PKCμ protein.
    Clone
    D22N3
    Synonym(s)
    Serine/threonine-protein kinase D1; Protein kinase C mu; Protein kinase D; nPKC-D1; nPKC-mu; PRKD1; PKD; PKD1; PRKCM
    Background
    Protein Kinase D (PKD) isoforms, including PKD1 (PKCμ), PKD2, and PKD3, are serine/threonine kinases involved in a variety of cellular processes regulated by PKC-dependent phosphorylation. PKDs have a conserved architecture with an N-terminal regulatory region containing two cysteine-rich C1 domains that bind diacylglycerol (DAG), and a pleckstrin homology (PH) domain, which exerts autoinhibition over their catalytic C-terminal kinase domain. Activation of PKD isoforms requires phosphorylation of two conserved serine residues in the activation loop (Ser744 and Ser748 for PKD1), mainly by PKCs. Additionally, PKD autophosphorylates at the C-terminal serine (Ser916 in PKD1), which correlates with increased catalytic activity. PKD isoforms regulate secretion, gene expression, cell proliferation, Golgi apparatus trafficking, muscle contraction, oxidative stress response, and play roles in signaling pathways related to cardiac hypertrophy, angiogenesis, and cancer progression. Protein kinase D (PKD) regulates cell migration, survival, and chemoresistance in part by phosphorylating class II histone deacetylases (HDACs), thereby modulating the epigenetic control of gene expression. Dysregulation of PKD activity is implicated in pathological conditions such as cancer and cardiovascular diseases.
    References
    • https://pubmed.ncbi.nlm.nih.gov/11943587/
    • https://pubmed.ncbi.nlm.nih.gov/28174535/

    Tech Support

    Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

    Handling Instructions

    Tel: +1-832-582-8158 Ext:3
    If you have any other enquiries, please leave a message.

    * Indicates a Required Field

    Please enter your name.
    Please enter your email. Please enter a valid email address.
    Please write something to us.