PKCβ Antibody [B15A17] | Primary Antibodies

Application: Reactivity: Human, Mouse, Rat

Lane 1: K562, Lane 2: Raji

Usage Information

Dilution
WB1:1000 IP1:50 FCM1:200

Application
WB, IP, FCM

Reactivity
Human, Mouse, Rat

Source
Rabbit Monoclonal Antibody

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
80 kDa

Datasheet & SDS

Biological Description

Specificity
PKCβ Antibody [B15A17] detects endogenous levels of total PKCβ protein.

Clone
B15A17

Synonym(s)
Protein kinase C beta type;PRKCB;PKCB;PRKCB1

Background
Protein kinase C β (PKCβ) is a conventional (classical) serine/threonine kinase isoform within the PKC family that is activated downstream of phospholipase C–coupled receptors and functions as a major node in diacylglycerol (DAG)‑ and Ca²⁺‑dependent signaling cascades controlling cell growth, survival, and differentiation. PKCβ is expressed as two closely related splice variants, PKCβI and PKCβII, which share nearly identical catalytic and regulatory domains but differ at their C‑terminal tails, leading to isoform‑specific localization and substrate preference. Upon activation by DAG, phosphatidylserine, and Ca²⁺, PKCβ translocates to membrane‑associated compartments where it phosphorylates a range of effectors, including MARCKS and other cytoskeletal regulators, and engages pathways linked to glucose transport, cytoskeletal reorganization, and inflammatory signaling. PKCβII is a key component of the B‑cell receptor (BCR) signaling axis, where it modulates B‑cell development, selection, and antibody output, and its overexpression can skew B‑cell subsets and elevate serum IgM. PKCβII is frequently implicated in promoting cell survival, epithelial–mesenchymal transition (EMT), and metastasis, partly through activation of NF‑κB and other pro‑survival transcriptional programs, and pharmacologic inhibition of PKCβ has been explored as a means to suppress tumor growth and chemoresistance in several solid‑tumor models.

Background

Protein kinase C β (PKCβ) is a conventional (classical) serine/threonine kinase isoform within the PKC family that is activated downstream of phospholipase C–coupled receptors and functions as a major node in diacylglycerol (DAG)‑ and Ca²⁺‑dependent signaling cascades controlling cell growth, survival, and differentiation. PKCβ is expressed as two closely related splice variants, PKCβI and PKCβII, which share nearly identical catalytic and regulatory domains but differ at their C‑terminal tails, leading to isoform‑specific localization and substrate preference. Upon activation by DAG, phosphatidylserine, and Ca²⁺, PKCβ translocates to membrane‑associated compartments where it phosphorylates a range of effectors, including MARCKS and other cytoskeletal regulators, and engages pathways linked to glucose transport, cytoskeletal reorganization, and inflammatory signaling. PKCβII is a key component of the B‑cell receptor (BCR) signaling axis, where it modulates B‑cell development, selection, and antibody output, and its overexpression can skew B‑cell subsets and elevate serum IgM. PKCβII is frequently implicated in promoting cell survival, epithelial–mesenchymal transition (EMT), and metastasis, partly through activation of NF‑κB and other pro‑survival transcriptional programs, and pharmacologic inhibition of PKCβ has been explored as a means to suppress tumor growth and chemoresistance in several solid‑tumor models.

References
https://pubmed.ncbi.nlm.nih.gov/20439192/ https://academic.oup.com/jb/article-abstract/132/5/677/919796

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%