Pim-1 Antibody [J10P8] | Primary Antibodies

Application: Reactivity: Human, Mouse

Usage Information

Dilution
WB1:1000

Application
WB

Reactivity
Human, Mouse

Source
Rabbit Monoclonal Antibody

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
34 kDa

Positive Control	Recombinant Pim-1, K562 cells; HEL cells; TF-1 cells; A20 cells
Negative Control

Datasheet & SDS

Biological Description

Specificity
Pim-1 Antibody [J10P8] detects endogenous levels of total Pim-1 protein.

Clone
J10P8

Synonym(s)
Serine/threonine-protein kinase pim-1; PIM1

Background
Pim-1 (Proviral integration site for Moloney murine leukemia virus-1) is a constitutively active serine/threonine kinase belonging to the PIM family, serving as an oncogene that promotes cell survival, proliferation, and tumorigenesis, particularly in hematologic malignancies and prostate cancer. It consists of an N-terminal lobe composed primarily of ??-sheets (residues 37–122) and a C-terminal lobe (residues 126–305) rich in ??-helichelices, featuring a unique hinge region (residues 123–125) with a proline residue that lacks a typical hydrogen bond donor, facilitating its role as a target for specific inhibitor design. The protein is expressed in two isoforms, a short form (Pim-1S) in the cytosol/nucleus and a long form (Pim-1L) at the plasma membrane, both of which lack a conventional regulatory domain. It is highly regulated by rapid turnover, and its activity is frequently induced by cytokine signaling through the JAK/STAT pathway. As a potent oncoprotein, it phosphorylates various substrates to inhibit apoptosis (Bad), promote cell cycle progression (p21, p27, Cdc25A), and stabilize transcription factors like c-Myc, often co-operating with it to drive oncogenesis, while also acting in drug resistance and enhancing tumor invasion.

Background

Pim-1 (Proviral integration site for Moloney murine leukemia virus-1) is a constitutively active serine/threonine kinase belonging to the PIM family, serving as an oncogene that promotes cell survival, proliferation, and tumorigenesis, particularly in hematologic malignancies and prostate cancer. It consists of an N-terminal lobe composed primarily of ??-sheets (residues 37–122) and a C-terminal lobe (residues 126–305) rich in ??-helichelices, featuring a unique hinge region (residues 123–125) with a proline residue that lacks a typical hydrogen bond donor, facilitating its role as a target for specific inhibitor design. The protein is expressed in two isoforms, a short form (Pim-1S) in the cytosol/nucleus and a long form (Pim-1L) at the plasma membrane, both of which lack a conventional regulatory domain. It is highly regulated by rapid turnover, and its activity is frequently induced by cytokine signaling through the JAK/STAT pathway. As a potent oncoprotein, it phosphorylates various substrates to inhibit apoptosis (Bad), promote cell cycle progression (p21, p27, Cdc25A), and stabilize transcription factors like c-Myc, often co-operating with it to drive oncogenesis, while also acting in drug resistance and enhancing tumor invasion.

References
https://pubmed.ncbi.nlm.nih.gov/16227208/ https://pubmed.ncbi.nlm.nih.gov/23936194/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%