Phospho-Tau (Ser214) Antibody [M8H2]

Application: Reactivity: Mouse, Human

Usage Information

Dilution
WB1:1000-1:10000 IHC1:100 - 1:250

Application
WB, IHC

Reactivity
Mouse, Human

Source
Rabbit Monoclonal Antibody

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
78 kDa 50-70 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Positive Control	Human cervical carcinoma tissue; Human brain tissue; Human normal kidney tissue; Human AD cerebral cortex tissue; Human normal spleen tissue; Mouse brain tissue; C57 mouse cerebral cortex; SH-SY5Y cells (Okadic acid and Calyculin A treated)
Negative Control	SH-SY5Y cells

Datasheet & SDS

Biological Description

Specificity
Phospho-Tau (Ser214) Antibody [M8H2] detects endogenous levels of total Tau protein only when it is phosphorylated at Ser214.

Clone
M8H2

Synonym(s)
MAPTL; MTBT1; TAU; MAPT; Microtubule-associated protein tau; Neurofibrillary tangle protein; Paired helical filament-tau; PHF-tau

Background
Phospho-Tau at serine 214 is a site-specifically phosphorylated form of the microtubule-associated protein Tau, occurring within the proline-rich region of its six isoforms and modified by kinases such as PKA, Akt, CDK5, GSK3 beta, and SGK1 to regulate cytoskeletal dynamics. The phosphorylation at Ser214 introduces a negative charge next to the KXGS motif, disrupting the interaction of Tau’s repeat domains with tubulin through electrostatic repulsion and enhancing binding to 14-3-3 proteins via a phospho-dependent motif that stabilizes soluble Tau. This modification causes hyperphosphorylated Tau to detach from microtubules, which prevents premature aggregation into paired helical filaments during development and stress, facilitates synaptic localization, and supports NMDA receptor-mediated currents by inhibiting Fyn kinase, helping to maintain axonal transport, neuronal excitability, and anti-apoptotic signaling. Phospho-Ser214 is strongly associated with early tau seeding activity in models of tauopathy, driving trans-synaptic propagation independently of classical phosphorylation markers, accumulating at synapses before neurofibrillary tangles, and promoting the pathological spread observed in Alzheimer’s disease and related dementias. Mutating this site to alanine eliminates seeding activity in neurons and Drosophila.

Background

Phospho-Tau at serine 214 is a site-specifically phosphorylated form of the microtubule-associated protein Tau, occurring within the proline-rich region of its six isoforms and modified by kinases such as PKA, Akt, CDK5, GSK3 beta, and SGK1 to regulate cytoskeletal dynamics. The phosphorylation at Ser214 introduces a negative charge next to the KXGS motif, disrupting the interaction of Tau’s repeat domains with tubulin through electrostatic repulsion and enhancing binding to 14-3-3 proteins via a phospho-dependent motif that stabilizes soluble Tau. This modification causes hyperphosphorylated Tau to detach from microtubules, which prevents premature aggregation into paired helical filaments during development and stress, facilitates synaptic localization, and supports NMDA receptor-mediated currents by inhibiting Fyn kinase, helping to maintain axonal transport, neuronal excitability, and anti-apoptotic signaling. Phospho-Ser214 is strongly associated with early tau seeding activity in models of tauopathy, driving trans-synaptic propagation independently of classical phosphorylation markers, accumulating at synapses before neurofibrillary tangles, and promoting the pathological spread observed in Alzheimer’s disease and related dementias. Mutating this site to alanine eliminates seeding activity in neurons and Drosophila.

References
https://pubmed.ncbi.nlm.nih.gov/19014373/ https://pubmed.ncbi.nlm.nih.gov/39211286/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%