Phospho-TAK1 (Ser439) Antibody [F17C11]

Application: Reactivity: Human

Lane 1: HeLa, Lane 2: HeLa (Calyculin A, 100nM; human IL-1β, 20ng/ml, 10 min), Lane 3: HeLa (Calyculin A, 100nM; human IL-1β, 20ng/ml, 10 min; alkaline phosphatase treated)

Usage Information

Dilution
WB1:1000 - 1:10000 IP1:10 - 1:100

Application
WB, IP

Reactivity
Human

Source
Rabbit Monoclonal Antibody

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
67 kDa 75 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Datasheet & SDS

Biological Description

Specificity
Phospho-TAK1 (Ser439) Antibody [F17C11] detects endogenous levels of total TAK1 protein only when it is phosphorylated at Ser439.

Clone
F17C11

Synonym(s)
TAK1; MAP3K7; Mitogen-activated protein kinase kinase kinase 7; Transforming growth factor-beta-activated kinase 1; TGF-beta-activated kinase 1

Background
Phospho-TAK1 (Ser439) is the activated form of transforming growth factor-β-activated kinase 1 (TAK1), a serine/threonine kinase encoded by MAP3K7 that assembles with regulatory subunits TAB1, TAB2, and TAB3 to coordinate stress-responsive intracellular signaling. TAK1 contains a kinase domain with critical activation loop residues and a C-terminal Ser439 site, whose autophosphorylation is essential for achieving full catalytic activity following initial ubiquitin-dependent activation. This modification stabilizes TAK1 and enhances its recruitment to polyubiquitin chains through TAB2 and TAB3, facilitating downstream phosphorylation events. Active TAK1 propagates signaling through multiple major pathways, including the NF-κB pathway via IKK complex phosphorylation, JNK and p38 MAPK cascades through MAP2K activation, and ERK signaling. These pathways collectively regulate innate immunity, inflammation, cell survival, apoptosis, embryonic development, and cellular responses to cytokines such as IL-1, TGF-β, TNF-α, and TLR ligands. Phosphorylation at Ser439 is particularly important for promoting TAK1-dependent activation of transcription factors like AP-1 and NF-κB, and dysregulation of this signaling node is implicated in inflammatory diseases, autoimmune disorders, and various cancers.

Background

Phospho-TAK1 (Ser439) is the activated form of transforming growth factor-β-activated kinase 1 (TAK1), a serine/threonine kinase encoded by MAP3K7 that assembles with regulatory subunits TAB1, TAB2, and TAB3 to coordinate stress-responsive intracellular signaling. TAK1 contains a kinase domain with critical activation loop residues and a C-terminal Ser439 site, whose autophosphorylation is essential for achieving full catalytic activity following initial ubiquitin-dependent activation. This modification stabilizes TAK1 and enhances its recruitment to polyubiquitin chains through TAB2 and TAB3, facilitating downstream phosphorylation events. Active TAK1 propagates signaling through multiple major pathways, including the NF-κB pathway via IKK complex phosphorylation, JNK and p38 MAPK cascades through MAP2K activation, and ERK signaling. These pathways collectively regulate innate immunity, inflammation, cell survival, apoptosis, embryonic development, and cellular responses to cytokines such as IL-1, TGF-β, TNF-α, and TLR ligands. Phosphorylation at Ser439 is particularly important for promoting TAK1-dependent activation of transcription factors like AP-1 and NF-κB, and dysregulation of this signaling node is implicated in inflammatory diseases, autoimmune disorders, and various cancers.

References
https://pubmed.ncbi.nlm.nih.gov/25028512/ https://pubmed.ncbi.nlm.nih.gov/33469458/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%