Home Primary Antibodies Phospho-STAT1 (Ser727) Antibody [F23C16]

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Phospho-STAT1 (Ser727) Antibody [F23C16]

Catalog No.: F2358

    Application: Reactivity:
    • F2358-wb
      Lane 1: U937 (IL-4 treated), Lane 2: U937

    Usage Information

    Dilution
    1:10000
    1:1000-1:100000
    Application
    WB, FCM
    Reactivity
    Human
    Source
    Rabbit Monoclonal Antibody
    Storage Buffer
    PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3
    Storage (from the date of receipt)
    -20°C (avoid freeze-thaw cycles), 2 years
    Predicted MW
    87 kDa

    Datasheet & SDS

    Biological Description

    Specificity
    Phospho-STAT1 (Ser727) Antibody [F23C16] detects endogenous levels of total STAT1 protein only when it is Phosphorylated at Ser727.
    Clone
    F23C16
    Synonym(s)
    Signal transducer and activator of transcription 1-alpha/beta; Transcription factor ISGF-3 components p91/p84; STAT1
    Background
    Phospho-STAT1 (Ser727) is the serine-phosphorylated form of STAT1 at residue Ser727, a modification that plays a pivotal role in regulating STAT1’s transcriptional activity within interferon (IFN) signaling. STAT1, a latent transcription factor, contains several domains: an N-terminal oligomerization domain, a coiled-coil domain, a DNA-binding domain, an SH2 domain for dimerization via Tyr701 phosphorylation, and a C-terminal transactivation domain (TAD) harboring the Ser727 phosphorylation motif (PMSP), which is targeted by MAPKs such as p38. Phosphorylation at Ser727 occurs downstream of IFN-α/γ, cellular stress, or LPS, dependent on prior Tyr701 activation, and enhances STAT1’s interaction with coactivators like CBP/p300. This modification is essential for maximal transcriptional activation of IFN-responsive genes: it augments promoter recruitment and chromatin binding, supporting antiviral responses, macrophage activation, and inflammation. Aberrant Ser727 phosphorylation is linked to tumorigenesis and impaired IFN signaling by disrupting normal STAT1 activity.
    References
    • https://pubmed.ncbi.nlm.nih.gov/18574148/
    • https://pubmed.ncbi.nlm.nih.gov/12671680/

    Tech Support

    Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

    Handling Instructions

    Tel: +1-832-582-8158 Ext:3
    If you have any other enquiries, please leave a message.

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