Home Primary Antibodies Phospho-SHP2 (Tyr542) Antibody [J17K6]

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Phospho-SHP2 (Tyr542) Antibody [J17K6]

Catalog No.: F2201

    Application: Reactivity:

    Usage Information

    Dilution
    1:1000
    1:30
    1:100 - 1:250
    Application
    WB, IP, IF
    Reactivity
    Mouse, Human
    Source
    Rabbit Monoclonal Antibody
    Storage Buffer
    PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3
    Storage (from the date of receipt)
    -20°C (avoid freeze-thaw cycles), 2 years
    Predicted MW Observed MW
    68 kDa 68 kDa
    *Why do the predicted and actual molecular weights differ?
    The following reasons may explain differences between the predicted and actual protein molecular weight.

    Datasheet & SDS

    Biological Description

    Specificity
    Phospho-SHP2 (Tyr542) Antibody [J17K6] detects endogenous levels of total SHP2 protein only when it is Phosphorylated at Tyr542.
    Clone
    J17K6
    Synonym(s)
    PTP2C; SHPTP2; PTPN11; Tyrosine-protein phosphatase non-receptor type 11; Protein-tyrosine phosphatase 1D; Protein-tyrosine phosphatase 2C; SH-PTP2; SH-PTP3; PTP-1D; PTP-2C; SHP-2; Shp2
    Background
    Phospho-SHP2 (Tyr542) refers to the tyrosine-phosphorylated form at residue Tyr542 of SHP2, also known as PTPN11, a non-receptor protein tyrosine phosphatase (PTP) belonging to the classical PTP family. SHP2 comprises two N-terminal SH2 domains (N-SH2 and C-SH2), a catalytic PTP domain, and C-terminal tyrosine residues including Tyr542 and Tyr580; phosphorylation at Tyr542 enables intramolecular interaction with the N-SH2 domain, relieving autoinhibition and exposing the active site. Phosphorylated Tyr542 also binds Grb2, acting as a scaffold to recruit signaling complexes independent of phosphatase activity. Phospho-SHP2 (Tyr542) activates upon growth factor stimulation via kinases like EphA2, promoting sustained ERK activation in Ras/MAPK pathways and modulating PI3K/AKT signaling through Gab1 dephosphorylation. It coordinates cellular responses to growth factors, cytokines, and hormones, regulating proliferation, differentiation, migration, and survival. Dysregulated Phospho-SHP2 (Tyr542) contributes to cancers via enhanced ERK signaling and to LEOPARD syndrome through aberrant MAPK activation.
    References
    • https://pubmed.ncbi.nlm.nih.gov/32395997/
    • https://pubmed.ncbi.nlm.nih.gov/23318428/

    Tech Support

    Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

    Handling Instructions

    Tel: +1-832-582-8158 Ext:3
    If you have any other enquiries, please leave a message.

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