Phospho-Rb (Ser780) Antibody [L13P18]

Catalog No.: F0501

For research use only.

    Home Primary Antibodies Phospho-Rb (Ser780) Antibody [L13P18]

    Application: Reactivity:

    Usage Information

    Dilution
    1:1000
    1:200
    Application
    WB, IP
    Reactivity
    Human, Mouse, Rat, Monkey
    Source
    Rabbit Monoclonal Antibody
    Storage Buffer
    PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3
    Storage (from the date of receipt)
    -20°C (avoid freeze-thaw cycles), 2 years
    Predicted MW
    110 kDa

    Datasheet & SDS

    Biological Description

    Specificity
    Phospho-Rb (Ser780) Antibody [L13P18] detects endogenous levels of Rb protein only when phosphorylated at Ser780.
    Clone
    L13P18
    Synonym(s)
    Retinoblastoma-associated protein; p105-Rb; p110-RB1; pRb (Rb); pp110; RB1
    Background
    Phospho-Rb (Ser780) is the phosphorylated, inactive form of the retinoblastoma protein (Rb), a key tumor suppressor and regulator of the G1/S cell cycle checkpoint. Rb contains three major domains—the N-terminal region, a central “pocket” domain critical for E2F transcription factor binding, and the C-terminal region—that together control its interactions with partner proteins. Phosphorylation at Ser780, located within the flexible linker region, is mediated by cyclin-dependent kinases (CDKs) during the G1/S transition. This phosphorylation induces a conformational change in Rb from an extended, active state to a compact, inactive form, thereby disrupting its ability to bind and inhibit E2F. As a result, E2F-driven transcription of genes required for DNA synthesis and cell cycle progression is unleashed, promoting proliferation. Importantly, detection of Rb phosphorylated at Ser780 is used as a functional marker of Rb inactivation, with clinical relevance in cancer prognosis, as loss of tumor-suppressive Rb activity through phosphorylation contributes to malignant progression.
    References
    • https://pubmed.ncbi.nlm.nih.gov/23516486/
    • https://pubmed.ncbi.nlm.nih.gov/23731755/

    Tech Support

    Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

    Handling Instructions

    Tel: +1-832-582-8158 Ext:3
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