Phospho-PLCγ1 (Tyr783) Antibody [C6N17]

Catalog No.: F0752

For research use only.

    Home Primary Antibodies Phospho-PLCγ1 (Tyr783) Antibody [C6N17]

    Application: Reactivity:

    Experiment Essentials

    WB
    Recommended SDS-PAGE separating gel concentration: 5%.

    Usage Information

    Dilution
    1:1000
    1:50
    1:100 - 1:400
    Application
    WB, IP, FCM
    Reactivity
    Human, Mouse
    Source
    Rabbit Monoclonal Antibody
    Storage Buffer
    PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3
    Storage (from the date of receipt)
    -20°C (avoid freeze-thaw cycles), 2 years
    Predicted MW
    155 kDa
    Positive Control NIH/3T3 cell (hPDGF-BB treated); A-431 cell (hEGF treated)
    Negative Control NIH/3T3 cell; A-431 cell

    Datasheet & SDS

    Biological Description

    Specificity
    Phospho-PLCγ1 (Tyr783) Antibody [C6N17] detetcs endogenous levels of PLCγ1 protein only when phosphorylated at Tyr783.
    Subcellular Location
    Cell projection
    Uniprot ID
    P19174
    Clone
    C6N17
    Synonym(s)
    phospholipase-C; phospholipase-C-gamma; PLC; PLC-g1; PLC-gamma; PLC-gamma-1; PLCg; PLCg1; PLCgamma; PLCgamma1
    Background
    Phospho-PLCγ1 (Tyr783) represents the activated form of PLCγ1, a receptor-proximal signaling enzyme that hydrolyzes phosphatidylinositol 4,5-bisphosphate (PIP₂) to generate the second messengers IP₃ and DAG, thereby activating protein kinase C (PKC) and mobilizing intracellular Ca²⁺. Structurally, PLCγ1 is composed of a catalytic core (X–Y domain) regulated by an autoinhibitory X–Y linker containing multiple domains: pleckstrin homology (PH), Src homology 2 (nSH2, cSH2), and SH3. In its basal state, the cSH2 domain interacts with the C2 domain of the catalytic core, physically blocking membrane access. PLCγ1 is broadly expressed in mammalian tissues, particularly in hematopoietic and epithelial cells, where it transduces signals downstream of receptor tyrosine kinases, immune receptors, and growth factor receptors. Phosphorylation at Tyr783, mediated by receptor-associated kinases, is the hallmark event that relieves autoinhibition: the phosphorylated Tyr783 acts as an intramolecular ligand that binds to the cSH2 domain, displacing it from the catalytic core and allowing exposure of a hydrophobic ridge required for membrane insertion and catalysis. This phosphorylation is essential because it locks PLCγ1 into its active conformation, stabilizes its interaction with the plasma membrane, and ensures robust propagation of receptor signaling, with dysregulated phospho-PLCγ1 implicated in oncogenesis and aberrant immune activation.
    References
    • https://pubmed.ncbi.nlm.nih.gov/35367415/
    • https://pubmed.ncbi.nlm.nih.gov/31889510/

    Tech Support

    Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

    Handling Instructions

    Tel: +1-832-582-8158 Ext:3
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